| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Hardee |
A | 8:22.01 | FHSAA State Track & Field Championships (2A/Para-Athlete) |
| 2 | Sebring |
A | 8:33.40 | FHSAA 3A District 7 |
| 3 | Avon Park |
A | 9:18.26 | FHSAA 2A District 9 |
| 4 | Lake Placid |
A | 9:33.80 | Avon Park Invitational |
| 5 | DeSoto County |
A | 9:35.99 | FHSAA 2A Region 3 |
| 6 | Okeechobee |
A | 9:40.23 | Okeechobee Running Series 2025 |
| 7 | Frostproof |
A | NT |