| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | LaBelle | B | Sandoval | Solis | Chairez | Wheatle | 56.21 | Lake Placid Meet 2 |
| 3 | DeSoto County | A | Nicklow | Henry | Campbell | Simons | 56.10 | Lake Placid Meet 2 |
| 4 | LaBelle | A | Collins | Esquivel | Garcia | Vasquez | 56.21 | Lake Placid Meet 2 |
| 5 | Lake Placid | A | Taylor | Walker | Madison | Madison | NT |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Avon Park | A | Brassell | Watts | Davis | Atkins | 54.81 | Lake Placid Meet 3 |
| 2 | Hardee | A | Tharp | Duran | Mushrush | Justice | 54.36 | FHSAA 2A Region 3 |
| 3 | Sebring | A | Davis | Brown | Ramberger | Hood | 50.42 | FHSAA 2A Region 3 |
| 4 | Clewiston | A | Arnold | Stephens | Bussott | Young | DNS | Glade Central Invitational 2023 |
| 5 | Okeechobee | A | Hancock | Lamb | Nesby | Bustos | 54.61 | Glades Central Raiders Invitational |