| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | WHITE, Sa'Niyah | DeSoto County |
NT | NT |
| 3 | ZAMORA, Yoselin | DeSoto County |
24.13 | Avon Park Meet #1 2025 |
| 4 | ADKINS-WILLIAMS, Destyne | Sebring |
NT | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | DESTINO, Zarmiah | Avon Park |
21.20 | Lake Placid Invite |
| 3 | JACKSON, Alexandra | Hardee |
19.64 | Larry Richardson Memorial Invitational |
| 4 | BOWES, Melina | Hardee |
21.02 | Mike Williams Classic @ Lake Region 2025 |
| 5 | PADRON, Anahi | DeSoto County |
21.46 | Immokalee Last Chance |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | PIERRE, Danaelle | Hardee |
18.74 | Larry Richardson Memorial Invitational |
| 3 | WATT, Kamila | Avon Park |
17.26 | Lake Placid Invite |
| 4 | JOHNSON, Mahagony | Avon Park |
17.63 | Lake Placid Invite |
| 5 | APONTE, Aya'na | Okeechobee |
19.07 | Royal Palm Beach Meet 2025 |