| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 1 | PETERSON, Madalynn |  Lake Placid | 
		NT | NT | 
| 2 | NUNEZ, Alexa |  Hardee | 
		NT | NT | 
| 3 | TINOCO, Jesselyn |  Hardee | 
		33.71 | Larry Richardson Memorial Invite | 
| 4 | MANRIQUEZ, Adela |  Hardee | 
		34.51 | HARDEE HS INVITATIONAL | 
| 5 | RAMIREZ, Michelle |  Clewiston | 
		NT | NT | 
| 6 | WITTER, Mickalia |  Clewiston | 
		NT | NT | 
| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 1 | CORTES, Ana |  Lake Placid | 
		32.48 | Dragons Invitational | 
| 2 | JOHNSON, Ariel |  DeSoto County | 
		31.83 | Lake Placid Meet #1 | 
| 3 | REED, Jada |  DeSoto County | 
		30.30 | Lake Placid Meet #3 | 
| 4 | ARONALD, Quanteria |  Moore Haven | 
		31.38 | North Port Invitational 2024 (Regional Qualifier) | 
| 5 | LETHBRIDGE, Meghan |  Sebring | 
		31.94 | Dragons Invitational | 
| 6 | JARVIS, Zarionna |  DeSoto County | 
		33.00 | Lake Placid Meet #3 | 
| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 1 | DESTINO, Zaymiah |  Avon Park | 
		29.76 | Lake Placid Meet #3 | 
| 2 | DILLON, Mykala |  Lake Placid | 
		29.54 | HARDEE HS INVITATIONAL | 
| 3 | THOMAS, Adore |  Moore Haven | 
		29.28 | Guy Thomas Memorial Classic (FHSAA & NFHS Sanctioned) | 
| 4 | HERRERA, Elissa |  Moore Haven | 
		29.42 | Lake Placid Meet #1 | 
| 5 | HOLLEY, Jaeda |  Hardee | 
		29.67 | Frostproof - The Ridge Open 2024 | 
| 6 | JOHNSON, Shamera |  Lake Placid | 
		30.26 | Lake Placid Meet #2 | 
| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 1 | LAMPKIN, Terrini |  Clewiston | 
		29.20 | Clewiston Big Lake Challenge 2024 | 
| 2 | WILLIAMS, Destyne |  Sebring | 
		27.74 | Dragons Invitational | 
| 3 | LING, Akeelah |  Moore Haven | 
		26.01 | Bishop Verot - Viking Elite Classic (Regional Qualifier) | 
| 4 | HAWTHORNE, Ramiya |  Sebring | 
		26.98 | Lake Placid Meet #3 | 
| 5 | GLABACH, Myra |  Avon Park | 
		28.17 | Lake Placid Meet #1 | 
| 6 | MARINE, Shelby |  Sebring | 
		29.25 | Clewiston Big Lake Challenge 2024 |