| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Fort Pierce Central |
A | Paul | Blue | Romelus | Benjamin | 4:27.76 | Cobra Relays 2026 |
| 4 | Martin County |
A | Hopkins | Lindsay | Barton | Osdon | 4:21.59 | Cobra Relays 2026 |
| 5 | West Boca Raton |
A | Sumpter-Henderson | Whitfield | Narvaez | Julien-Jeune | 4:23.29 | Palm Beach Gardens Invite #1 2026 |
| 6 | Jensen Beach |
A | Acheampong | Melda | Peruski | Laurent | DNS | Jensen Beach Meet #2 |