| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | FREDERIC, Amon | Tradition Prep | NT | NT |
| 3 | MCMILLER, Zayd | Fort Pierce Central |
NT | NT |
| 4 | HARRISON, Brian | John Carroll |
1:03.64 | Benjamin Invite #1 2025 (Sanctioned) |
| 5 | ROBERTS, Jaden | Fort Pierce Westwood |
NT | 55.69 |
| 6 | WHITTICK, Nyheiim | Somerset College Prep | NT | NT |
| 7 | JEAN, Frantzner | Fort Pierce Westwood |
NT | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | BRADFORD, Jonathon | Somerset College Prep | 58.39 | Wellington HS Invitational |
| 2 | RIVERON, Adrian | Tradition Prep | 57.83 | Atlantic HS Invitational 2 |
| 3 | BARBER, Kameron | Centennial |
56.66 | Palm Bay Invitational |
| 4 | FILSAINE, Don | Fort Pierce Central |
56.17 | MCHS Fesh/Soph 2025 |
| 5 | LOPEZ, Ethan | Tradition Prep | 56.18 | Atlantic HS Invitational 2 |
| 6 | ALBERT, Vladimir | Port St. Lucie |
57.51 | Rockledge Raider Classic |
| 7 | HORNE, David | Somerset College Prep | 58.00 | PBGHS Invitational 2025 |
| 8 | PARFAIT, Guerry | Lincoln Park |
1:00.45 | Bill Wilson Invitational 2025 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | ESTASSIN, Jayden | Treasure Coast | 55.50 | Jupiter Warrior Invitational 2025 |
| 2 | RICHARDSON, Antonio | Port St. Lucie |
53.89 | Astronaut Season Opener 2025 |
| 3 | FLORES, Alexio | Port St. Lucie |
53.43 | Rockledge Raider Classic |
| 4 | MONACO, Franco | John Carroll |
52.39 | Bill Wilson Invitational 2025 |
| 5 | ROSICHAN NADER, Jacob | Lincoln Park |
52.78 | Bill Wilson Invitational 2025 |
| 6 | DRUMMOND, Jahzion | Tradition Prep | 53.66 | Atlantic HS Invitational 2 |
| 7 | NATHAN-MARSH JR, Alexander | Fort Pierce Central |
54.96 | Palm Bay Invitational |
| 8 | PARKER, D'Marcus | Port St. Lucie |
55.73 | Bulldog Invitational 2025 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | DELEON, Steven | Fort Pierce Central |
51.46 | Palm Bay Invitational |
| 2 | HILL, Michael | Somerset College Prep | 50.91 | PBGHS Invitational 2025 |
| 3 | ENOCH, Melik | Centennial |
49.56 | Bill Wilson Invitational 2025 |
| 4 | JEAN-PIERRE, Giovani | Centennial |
47.11 | Bill Wilson Invitational 2025 |
| 5 | BRADLEY, James | John Carroll |
49.16 | Bill Wilson Invitational 2025 |
| 6 | HARPER, Andrew | Treasure Coast | 49.98 | Kings Academy Varsity 3 Sanctioned |
| 7 | JOHNS, Marc | Centennial |
51.11 | Dr. Garcia Invite #2 2025 |
| 8 | JONES, Bryan | Treasure Coast | 52.37 | Park Vista #1 2025 |