| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | 
|---|---|---|---|---|---|---|---|
| 3 | Naples Christian Academy | B | Pierre | Osthoff | Sawyer | Break | 1:07.57 | 
| 4 | Village School - Lower School | A | Weisbecker | Slattery | Michie | Shea | 1:14.11 | 
| 5 | St. Elizabeth Seton School | B | Rady-Pantek | Hernandez | Boone | Rubin | 1:11.35 | 
| 6 | Village School - Lower School | B | Reinoso | Mauceri | Josephs | Hartman | 1:14.11 | 
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | 
|---|---|---|---|---|---|---|---|
| 2 |  Donahue Academy | 
		B | Osorio | Trabbic | Umana | Yates | 1:14.00 | 
| 3 | Naples Christian Academy | A | Piazza | Weng | Castro | Meistrell | 1:07.57 | 
| 4 |  Donahue Academy | 
		A | Halisky | Graham | Green | Patterson | 1:04.00 | 
| 5 | St. Anns | A | Le Quere | Matusz | Ho | Day | 1:04.00 | 
| 6 | Royal Palm Academy | A | Teet | Andrade | Waller | Lewis | 1:21.00 | 
| 7 | St. Elizabeth Seton School | A | Truby | Kleiber | Hyppolite | Manfredi | 1:11.35 |