| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 3 | Village School - Lower School | B | Evans | Lane | Miranti | Pietzak | NT |
| 4 | Donahue Academy |
B | Freeze | Trabbic | Yates | Minter | 5:25.00 |
| 5 | Naples Christian Academy | B | Weng | Meistrell | Snyder | Simon | NT |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Naples Christian Academy | A | Castro | Morisson | Alonso | Sawyer | NT |
| 3 | St. Elizabeth Seton School | A | Cronin | Scuderi | Conlon | Manfredi | NT |
| 4 | Donahue Academy |
A | Webb | Webb | Watson | Graham | 4:42.63 |
| 5 | St. Anns | A | Day | Bijou | Wasser | Burke | 5:00.00 |
| 6 | Royal Palm Academy | A | O'Malley | Mulligan | Schuetz | Waller | 5:41.00 |
| 7 | Village School - Lower School | A | Reinoso | Baschieri | Kaczynski | Slattery | NT |