| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Village School - Lower School | B | Josephs | Reinoso | Kaczynski | Hartman | NT |
| 3 | St. Elizabeth Seton School | B | Fuller | Cronin | Ashby | Boone | NT |
| 4 | Donahue Academy |
B | Minter | Trabbic | Freeze | Yates | 1:14.00 |
| 5 | Naples Christian Academy | B | Pierre | Osthoff | Sawyer | Leeper | NT |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 2 | Donahue Academy |
A | Graham | Osorio | Green | Patterson | DQ |
| 3 | Naples Christian Academy | A | Piazza | Weng | Castro | Meistrell | NT |
| 4 | St. Anns | A | Le Quere | Matusz | Ho | Day | 1:04.00 |
| 5 | Royal Palm Academy | A | Andrade | Waller | Pacheco | Lewis | 1:08.00 |
| 6 | St. Elizabeth Seton School | A | Conlon | Hyppolite | Truby | Manfredi | NT |
| 7 | Village School - Lower School | A | Weisbecker | Slattery | Baschieri | Michie | NT |