| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Naples Christian Academy | A | Weng | Casto | Abbott | Meistrell | NT |
| 2 | St. Elizabeth Seton School | A | Styrfflyer | Nieves | Elarde | Bolonas | NT |
| 3 | Royal Palm Academy | A | Waller | Zairis | Barber | Mulligan | 6:00.00 |
| 4 | Donahue Academy |
A | Copeland | Graham | Adamic | Roder | 3:47.65 |
| 5 | Village School - Lower School | A | Lamb | DeFont | Guidone | Siddiqi | NT |
| 6 | Donahue Academy |
B | Romero | Martinez | McCabe | Trabbic | 5:00.00 |
| 7 | Royal Palm Academy | B | Finnegan | Gavin | Pell | Camacho | 6:34.00 |