| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | St. Elizabeth Seton School | A | Helzel-Breen | Elarde | McQuade | Styrfflyer | NT | |
| 3 | Royal Palm Academy | A | Mulligan | Jimenez | Murphy | Barber | 5:15.00 | |
| 4 | Donahue Academy | A | Copeland | Copeland | Estes | Lebrun | 3:50.76 | 3:50.76 Late Season Dual Meet NHS %26 Gulf Coast |
| 5 | St. Anns | A | McPhillips | McPhillips | Burke | Matusz | 5:00.00 | |
| 6 | Village School - Lower School | A | Germain | Lapato | Nocera | Bidwell | NT | |
| 7 | Naples Christian Academy | A | Weng | Weng | Piazza | Casto | NT | |
| 8 | Village School - Lower School | B | NT |