| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | St. Elizabeth Seton School | A | 5:30.00 | Unverified: | ||||
| 3 | Village School of Naples | C | Farrugia | Siddiqi | Park | Lapato | 4:07.13 | Verified |
| 4 | Village School of Naples | B | Egan | Chapman | Boone | Germain | 4:07.13 | Verified |
| 5 | Village School of Naples | A | Clegg | Amos | Witt | Park | 4:07.13 | Verified |
| 6 | Donahue Academy of Ave Maria | A | Kadlec | Allen | Joyce | Borjon | 4:48.00 | Verified |
| 7 | St. Elizabeth Seton School | B | DNS | Unverified: |