| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Dreyfoos |
A | Marshall | Vedieu | Jackson | Perez | DNS | FHSAA 2A District 8 |
| 4 | Lake Worth |
A | Robinson | Christian | Buchanan | LaJeune | 44.75 | Jeff Fossen Invitational 2025 |
| 5 | Seminole Ridge |
A | Wellons | Neal | Wingate | Allen | 45.00 | |
| 6 | Port St. Lucie |
A | Joubert | Davis | Tavarez | Pata | DNS | Jensen Beach Invitational 2026 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Royal Palm Beach |
A | Rolle | Williams | Thomas | Curry | 43.63 | Royal Palm Beach Meet 2025 |
| 2 | Boynton Beach |
A | Middleton | Ware-Roberson | King | Espady | 43.40 | Boynton Beach HS Small Meet |
| 3 | Palm Beach Gardens |
A | Derilus | Campbell | Bennett | Coicou | 42.45 | FHSAA 4A District 8 |
| 4 | Cardinal Newman |
A | Gantt | Elam | Mcclellion | Labode | DNS | Harry Howell Invitational Hosted by Cardinal Newman (Qualifi |
| 5 | Santaluces |
A | Gerald | Toussaint | Zennot | Prince | 42.25 | PBC Meet 2025 |
| 6 | Pahokee |
A | Simms | Fitzgerald | Stinfort | Hill | 42.45 | FHSAA 1A Region 4 |
| 7 | Jupiter |
A | Martin | Peters | Havasi | Garabrant | 43.56 | FHSAA 4A District 8 |
| 8 | Suncoast |
A | Phillips | Caple | Saintil | Normil | 44.62 | Royal Palm Beach Invitational 2025 |