| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Suncoast |
A | Louis | Hopkins | Felin | Johnson | NT | |
| 4 | Seminole Ridge |
A | Juntunen | Worrell | Coffey | Langsi | 52.66 | Welington Meet #1 |
| 5 | John I. Leonard |
A | louis | toussaint | barrett | castillo | 54.32 | John I Leonard Invitational 1 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Wellington |
A | Lowe | Saintfort | Carbocci | Richards | 51.52 | Wellington HS Invitational |
| 3 | Lake Worth |
A | Thompson | Jones | Razz-Wilkins | Razz | 50.32 | Royal Palm Beach Invitational 2025 |
| 4 | Palm Beach Gardens |
A | Mcgee | Perry | Collier | Hooks | 49.09 | FHSAA 4A Region 2 |
| 5 | Pahokee |
A | Jones | White | Washington | Johnson | 50.11 | FHSAA 1A Region 4 |
| 6 | Glades Central |
A | Forbes | Tibo | Marshall | Butts | 51.44 | Glades Central Invite #1 2025 |
| 7 | Dreyfoos |
A | Vasallo | Mcdow | Pierre | Cannon | 52.33 | John I Leonard Invitational 1 |