| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Jupiter |
A | Hillberg | Roberts | Hawken | Shaw | 9:50.93 | FHSAA 4A Region 2 |
| 2 | Cardinal Newman |
A | Brown | D'Angelo | Mccartney | Veillard | 10:12.16 | Louie Bing Invitational |
| 3 | Royal Palm Beach |
A | Chapman | Desravins | Neptune | Ennis | 11:05.41 | FHSAA 4A Region 2 |
| 4 | Boynton Beach |
A | Allen | Jean | Francis | Isidor | 11:05.43 | PBC Meet 2025 |
| 5 | Glades Central |
A | Frazier | Roa | Roman | Roa | 12:00.69 | Pahokee Anquan Boldin Invitational 2025 |
| 6 | Seminole Ridge |
A | McNish | Duke | Gillis | Alvin | 12:06.94 | John I Leonard Invitational 1 |
| 7 | Palm Beach Gardens |
A | Ware | Hall | Garcia | Gaines | 12:52.77 | Harry Howell Invitational Hosted by Cardinal Newman (Qualifi |
| 8 | Dreyfoos |
A | McDow | Carbo | Garmley | Guillett | 13:02.25 | St Andrews Varsity Invite 1 |