| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Palm Beach Lakes |
A | Stevenson | Daniels | Stevenson | Charles | 4:05.00 | |
| 2 | Dreyfoos |
A | Roby | Gou | Satterwhite | Aquel-Lacayo | DNS | St Andrews Varsity 3 |
| 3 | John I. Leonard |
A | Joseph | gerilus | nelson | louis | 3:45.60 | John I Leonard Invitational 1 |
| 4 | Boynton Beach |
A | Cleus | Middleton | Morgan | Dufren | 3:40.96 | Blanche Ely Meet March 04 |
| 5 | Lake Worth |
A | Pierre-Louis | Frazil | Florexio | Christian | DNS | Royal Palm Beach Invitational 2025 |
| 6 | Okeechobee |
A | Evans | Summerford | Sears | Stewart | 3:53.71 | Rockledge Raider Classic |
| 7 | Seminole Ridge |
A | Charleston | Linton | Archambault | Allen | 4:02.11 | |
| 8 | Suncoast |
A | Aklil | Okoh | Saintil | Xue | NT |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Port St. Lucie |
A | Mayberry | Barnwell | Davis | Wright | 3:35.05 | MCHS Sarge Invitational 2025 |
| 2 | Palm Beach Gardens |
A | Campbell | Jean-Baptiste | Bennett | Coicou | 3:24.30 | FHSAA 4A Region 2 |
| 3 | Glades Central |
A | Baptist | Charlemond | Goring | Israel | 3:22.26 | FHSAA State Track & Field Championships (2A/Para-Athlete) |
| 4 | Cardinal Newman |
A | Coleman | Burgoon | Watkins | Alexander | 3:17.82 | Florida Relays |
| 5 | Santaluces |
A | Gerald | Sanon | Ganiev | Desir | DNS | FHSAA State Track & Field Championships (4A) |
| 6 | Pahokee |
A | Logan | Grantlin | Morales | Jones | 3:22.80 | FHSAA State Track & Field Championships (1A) |
| 7 | Royal Palm Beach |
A | Curry | Bowleg | Greenfield | Civil | 3:28.95 | FHSAA 4A District 8 |
| 8 | Wellington |
A | Borgella | Davis | Guido | Pierre-Louis | 3:36.00 |