| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Cardinal Newman |
A | Wagner | Stephenson | Simpson | Licata | 7:43.78 | Florida Relays |
| 2 | Jupiter |
A | McCarthy | Diaz | O'Shea | Ward | 8:30.60 | FHSAA 4A District 8 |
| 3 | Royal Palm Beach |
A | Girard | Caldovino | Baker | Kelly | 8:34.83 | FHSAA 4A District 8 |
| 4 | Pahokee |
A | Cheves | Roman | Boyce | Watson | 8:59.61 | Jeff Fossen Invitational 2025 |
| 5 | Wellington |
A | Hoover | Mundo | Prospere | Reyka | 9:00.00 | |
| 6 | Dreyfoos |
A | Gou | Tidwell | Riche | Turecki | 9:00.20 | FHSAA 2A Region 2 |
| 7 | Palm Beach Lakes |
A | De LaRosa | Stevenson | Daniels | Stevenson | 9:06.62 | Harry Howell Invitational Hosted by Cardinal Newman (Qualifi |
| 8 | Palm Beach Gardens |
A | McRae | Jean Baptiste | Josey | Mompoint | 9:17.87 | The Lincoln Knowles Invitational 2025 |
| 9 | Santaluces |
A | Morrison | Abelland | Joseph | Daniel | 9:25.05 | Panther Relays 2025 |
| 10 | Glades Central |
A | Georges | Ramirez | Ramirez | Bryant | 9:28.25 | FHSAA 2A District 8 |
| 11 | Okeechobee |
A | Phillips | Jaimes | Spencer | Zuniga | 9:40.23 | Okeechobee Running Series 2025 |
| 12 | John I. Leonard |
A | rodriguez | nelson | Joseph | wilson | DNS | Welington Meet #1 |
| 13 | Lake Worth |
A | Gilead | Santiago | Charles | Louinelson | 9:52.49 | Harry Howell Invitational Hosted by Cardinal Newman (Qualifi |
| 14 | Seminole Ridge |
A | Linton | Hughes | Grissett | Reyes | 9:55.00 | |
| 15 | Suncoast |
A | Jolicoeur | DeStefano | Aklil | Auguste | NT |