| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Seminole Ridge |
A | Coffey | Jean | Davies | Langsi | 54.23 | Dr. Garcia Invite #2 2025 |
| 4 | Palm Beach Central |
A | Hendrix | Ross | Turner | Perez | 52.88 | Atlantic HS Invitational 2 |
| 5 | Dreyfoos |
A | Cannon | Pierre | Pierre | Mcdow | 53.54 | Royal Palm Beach Meet 2025 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Inlet Grove |
A | Sharpe | Steward | Harper | Leveille | DNS | PBC Meet 2025 |
| 3 | Lake Worth |
A | Clayton | Jones | Greenwich | Razz | 50.97 | Cardinal Newman Invitational 2025 |
| 4 | Somerset Canyons |
A | Roache | Demosthene | Pierre-Paul | Bradley | 50.61 | Royal Palm Beach Meet 2025 |
| 5 | Suncoast |
A | Brown | Johnson | Louis | Hester | 50.69 | Benjamin Invite #1 2025 (Sanctioned) |
| 6 | Pahokee |
A | Jones | Johnson | White | Washington | 51.14 | Glades Central Invite #1 2025 |
| 7 | Wellington |
A | Brooks | Carbocci | Ramirez | Suniga | DNS | Wellington HS Invitational |