| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 2 | Seminole Ridge |
A | Alvin | Prince | Allen | Gillis | DNS | John I Leonard Invitational |
| 4 | Somerset Canyons |
A | Hills | Leger | Senat | Garcia | DNS | |
| 6 | Inlet Grove |
A | Steward | Sharpe | Harper | Gibson | DNS | |
| 7 | Boynton Beach |
A | Denmark | Francis | Jean | Avila | DNS | John I Leonard Invitational |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Royal Palm Beach |
A | sainvil | Bradwell | Davis | Neptune | NT | |
| 2 | Palm Beach Central |
A | Fabinger | Anderson | Ter-Avanesova | Chin-Fo-Sieeuw | 4:36.84 | Dr Garcia Invite #1 2025 |
| 3 | Palm Beach Gardens |
A | Higgins | Price | Bamberry | Perry | 4:27.01 | |
| 4 | American Heritage (DB) |
A | Milord | Watts | Cage | Johnson | 4:12.00 | |
| 5 | Dreyfoos |
A | Cannon | Vasallo | Mcdow | Pierre | 4:26.85 | Dr Garcia Invite #1 2025 |
| 6 | Wellington |
A | Roberts | Bentley | Carbocci | Brooks | 4:30.39 | Spanish River Invitational 2025 |
| 7 | Centennial |
A | Schmook | Robinson | Mitton | Hoskins | NT | |
| 8 | Suncoast |
A | Anim Addo | Lewis | Lawrence | Felin | 5:16.19 | John I Leonard Invitational |