| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Seminole Ridge |
A | caporizzo | Alvin | Leslie | Carrera | NT | |
| 2 | American Heritage (DB) |
A | Kiyota Ayrosa | Oliscat | Steele | St Julien | NT | |
| 3 | Centennial |
A | Jean-Pierre | Enoch | Dimarco | Kaegi | NT | |
| 4 | Suncoast |
A | Phillips | Menardy | Gordon | Morant | 4:03.74 | John I Leonard Invitational |
| 5 | Dreyfoos |
A | Roby | Fischetti | Aquel-Lacayo | Joseph | DNS | Kings Academy Varsity 1 |
| 6 | Palm Beach Central |
A | Ledford | Lindsey-Hartwig | Augustin | Atkins | NT | |
| 7 | Okeechobee |
A | Santos | Summerford | Immerfall | Stewart | NT |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Boynton Beach |
A | Cleus | Humphreys | Holliday | Telisca | DNS | John I Leonard Invitational |
| 2 | Palm Beach Gardens |
A | Baker | Robinson | Coicou | Voltaire | 3:39.04 | John I Leonard Invitational |
| 3 | Wellington |
A | Smith | Spence | Wroy | Paul | 3:34.25 | Spanish River Invitational 2025 |
| 4 | Royal Palm Beach |
A | Williams | Murvin | Smith | Thomas | 3:30.45 | |
| 5 | Santaluces |
A | Sanon | Michel | Gerald | Zennot | 3:31.37 | John I Leonard Invitational |
| 6 | Inlet Grove |
A | Grantlin | Francis | Francis | Man | 3:36.68 | John I Leonard Invitational |
| 7 | Cardinal Newman |
A | Curry | Thelemaque | Thelemaque | Hart | DNS | |
| 8 | Somerset Canyons |
A | Riley | Dumerin | Griffiths | David | DNS | John I Leonard Invitational |