| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | DEMOSTHENE, Jenise | Atlantic (Delray) |
NT | |
| 2 | RICHARDSON, Ai'yanna | Dwyer |
NT | |
| 3 | SAMAYOA, Gisselle | Seminole Ridge |
22.22 | Lincoln Knowles Invitational 2024 (FHSAA Sanctioned) |
| 4 | YEPES, Isabella | Park Vista |
21.61 | FHSAA 4A District 12 |
| 5 | SUMPTER-HENDERSON, Dominique | West Boca Raton |
22.22 | John I Leonard Invitational |
| 6 | FRANKLIN, Raegan | Dwyer |
22.61 | Dwyer Panther Open 2025 |
| 7 | SANCHEZ, Joanna | Park Vista |
NT | |
| 8 | PETIT-HOMME, Jalah | Dr. Joaquin Garcia | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | ALLEN, Hailey | Seminole Ridge |
19.68 | |
| 2 | JOLY, Eleonore | Park Vista |
18.92 | FHSAA 4A Region 3 |
| 3 | GAY, Autumn | Dwyer |
17.72 | Dr Garcia Invite #1 2025 |
| 4 | ODITA, Katherine | Dwyer |
16.08 | Dwyer Panther Open 2025 |
| 5 | SAMA, Arianna | Treasure Coast | 16.71 | FHSAA 4A District 10 |
| 6 | MASSENA, Chrislaine | Park Vista |
18.07 | Palm Beach County Championships |
| 7 | WALTERS, Kiara | Treasure Coast | 19.65 | FHSAA 4A District 10 |
| 8 | WOODS, Bailey | Atlantic (Delray) |
20.97 | FHSAA 3A District 14 |