| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | A | Villegas | Ramsey | Williams | Brown | DNS | ||
| 3 | King's Academy |
A | Spicer | George | Minikus | Storey | 1:25.89 | Panther Relays 2025 |
| 5 | Dwyer |
A | Kjos | Sturgis | Franklin | Bronson | 1:08.82 | Panther Relays 2025 |
| 7 | Coconut Creek |
A | Cohen | Bartley | Escarment | Cooper | DNS |