| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Dwyer |
B | Bradshaw | Olofin | Lewis | Reed | NT | |
| 4 | Jupiter |
A | Rebimbas | Dettman | Click | Musil | NT | |
| 5 | McArthur |
A | Gilkes | Gourdine | Grant | louis | DNS |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Wellington |
A | NT | |||||
| 2 | Boca Raton |
A | Geathers | Buchanan | Hails | Buendia | NT | |
| 3 | Lake Worth Christian |
A | Collado | Davidson | Rios | Dominique | 1:53.80 | Panther Relays 2025 |
| 4 | A | Charlot | McKay | Oscar | Vilma | 1:38.82 | Panther Relays 2025 | |
| 5 | Atlantic Christian |
A | Enamorado | Belfort | Exume | James | 1:48.11 | Panther Relays 2025 |
| 6 | Jupiter Christian |
A | Mirabella | Beard | Brandon | Zuniga | NT | |
| 7 | Clewiston |
A | Allen | Perez | Givens | Vasquez | NT |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Coconut Creek |
A | Lamar | Weekes | Buggam | Miller | 1:34.67 | Louie Bing Invitational |
| 2 | Dwyer |
A | Brown | Feaman | Martinez | Wilson | 1:33.37 | Louie Bing Invitational |
| 3 | Oxbridge Academy |
A | Patterson | Vax | Frederick | Pribramsky | DNS | |
| 4 | Cardinal Newman |
A | Hart | Gantt | Sanders | Elam | 1:25.27 | Louie Bing Invitational |
| 5 | Park Vista |
A | Perkins | Vittori | Villalba | Gamble | 1:32.46 | Louie Bing Invitational |
| 6 | Cypress Bay |
A | Barnett | Barnett | Claude | Sarmiento | DNS | Louie Bing Invitational |
| 7 | King's Academy |
A | Ruidiaz | Tome | Driggers | Beneby | 1:34.45 | Panther Relays 2025 |
| 8 | Suncoast |
A | Normil | Phillips | Caple | Ward | 1:36.75 | Panther Relays 2025 |