| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Oxbridge Academy |
A | Merida | Conroy | Garine | Herrera | DNS | |
| 2 | Clewiston |
A | Bryant | Dixon | Sainz | Givens | 3:39.85 | FHSAA 2A District 12 |
| 3 | Suncoast |
A | Moore | Johnson | Depp | Yazar | 3:36.23 | Royal Palm Beach Invitational 2025 |
| 4 | Coconut Creek |
A | Miller | Norcine | Pluviose | Buggam | 3:31.23 | 2025 The Ellis Elite 16 Track and Field Invitational |
| 5 | McArthur |
A | Lloyd | Jones | Wright | Walker | DNS | FHSAA 3A Region 4 |
| 6 | Boca Raton |
A | Wittenberg | Elk | Flores | Giraro | 3:37.07 | FHSAA 4A District 13 |
| 7 | Jupiter Christian |
A | Brandon | Franco | Penner | Evans | 3:45.21 | FHSAA 1A Region 4 |
| 8 | Lake Worth Christian |
A | Davidson | Rios | Collado | Gould | DNS | Clyde Harris Invitational at |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Park Vista |
A | Jones | Paul | Jimmy | Colt | 3:29.02 | FHSAA 4A District 13 |
| 2 | Wellington |
A | Davis | Paul | Guido | Borgella | DNS | FHSAA 4A District 13 |
| 3 | Dwyer |
B | Moore | Dutkewych | Farelo | Fresneda-Molina | 3:24.28 | FHSAA 4A Region 2 |
| 4 | Cardinal Newman |
A | Simms | Newby | Watkins | Alexander | 3:17.82 | Florida Relays |
| 5 | King's Academy |
A | Daboh | Guerrero | Powell III | Kakuk | 3:23.77 | FHSAA 2A District 8 |
| 6 | Dwyer |
A | Quince | Martinez | Williams | Wilson | 3:24.28 | FHSAA 4A Region 2 |
| 7 | Atlantic Christian |
A | Arterburn | James | Exume | Enamorado | DNS | FHSAA State Track & Field Championships (1A) |
| 8 | A | Robinson | Gilchrist | Checo | Rheingold | 3:30.14 | FHSAA 3A Region 4 |