| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Wellington |
A | Hoover | Wilson | Mundo | Arram | 11:21.00 | |
| 2 | Park Vista |
A | Sorgente | Jimmy | Colt | Guyett | 11:24.89 | Panther Relays 2025 |
| 3 | King's Academy |
A | Bertolami | Crowley | Guerrero | Gast | 11:45.68 | Panther Relays 2025 |
| 4 | A | Vertefeuille | Gilchrist | Dieguez | Roman | 12:07.11 | Panther Relays 2025 | |
| 5 | Boca Raton |
A | Kotelnikov | Mayen | Villedrouin | Cancino | 12:09.20 | Dr. Joaquin Garcia Bulldog Distance Jamboree 2025 |
| 6 | Jupiter Christian |
A | Stanek | Evans | Gilliam | FLORA | 12:19.94 | WA Relays |
| 7 | Suncoast |
A | Kelly | Boles | Campbell | Streets | 13:27.17 | Panther Relays 2025 |
| 8 | Dwyer |
A | Sandala | Kamaras | Jefferson | Correa | NT | |
| 9 | Clewiston |
A | Bussott | mckenzie | mullings | Segura | NT | |
| 10 | Coconut Creek |
A | Alexis | Donaldson | Burns | Titre | NT | |
| 11 | Cardinal Newman |
A | Sanders | Licata | Stephenson | Simpson | NT | |
| 12 | McArthur |
A | Bertram | Novembre | Tifa | Boisvert | DNS | |
| 13 | Oxbridge Academy |
A | Jassenoff | Patterson | Wolff | Frackelton | DNS |