| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Suncoast |
A | NT | |
| 2 | Lake Worth Christian |
A | NT | |
| 3 | Somerset College Prep | A | 46.00 | |
| 4 | Palm Beach Central |
A | 44.71 | 44.71 Royal Palm Beach Invitational |
| 5 | Wellington |
A | 44.90 | |
| 6 | Dr. Joaquin Garcia | A | NT | |
| 7 | Park Vista |
A | NT |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | King's Academy |
A | 43.24 | FHSAA 2A Region 4 |
| 2 | Atlantic (Delray) |
A | DNS | |
| 3 | Santaluces |
A | 43.11 | |
| 4 | Seminole Ridge |
A | 42.82 | 42.82 Glade Central Invitational 2024 |
| 5 | Palm Beach Gardens |
A | 42.89 | |
| 6 | Benjamin School |
A | 43.17 | FHSAA State Track & Field Championships (1A) |
| 7 | Dwyer |
A | 43.21 | |
| 8 | Forest Hill |
A | 44.24 | 44.24 Warrior Invitational 2023 |