| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 3 | FOWLER, Ta'Niyah | Glades Central |
NT | 19.02 |
| 4 | PORTER, Heraje | Royal Palm Beach |
NT | NT |
| 5 | PINTO, Winnyia | Glades Central |
NT | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | RICHARDSON, Ai'yanna | Dwyer |
23.67 | Park Vista #1 2025 |
| 2 | POOLE, Paylyn | Glades Day |
21.01 | Pahokee Anquan Boldin Invitational 2025 |
| 3 | MOURA, Christianna | Atlantic Christian |
20.73 | Benjamin Invite #1 2025 (Sanctioned) |
| 4 | CAMPBELL, Evanique | Suncoast |
19.88 | Royal Palm Beach Meet 2025 |
| 5 | SCHROEDER, Natascha | Wellington |
19.92 | Royal Palm Beach Meet 2025 |
| 6 | ELBERT, Torrlasia | Palm Beach Gardens |
20.74 | Dwyer Panther Open 2025 |
| 7 | FRANKLIN, Raegan | Dwyer |
21.67 | Park Vista #1 2025 |
| 8 | MINUS, Lawrencia | Glades Central |
NT | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | BRONSON, Emma | Dwyer |
19.00 | Dwyer Panther Open 2025 |
| 2 | HESTER, Aaliyah | Suncoast |
18.44 | Benjamin Invite #1 2025 (Sanctioned) |
| 3 | BAILEY, Denijah | Glades Day |
18.10 | Pahokee Andre Powell Invitational |
| 4 | PATILLO, RoDajah | Santaluces |
16.10 | Louie Bing Invitational |
| 5 | BOURNE-MORGAN, Natasha | Somerset College Prep | 16.24 | Pahokee Andre Powell Invitational |
| 6 | ROBERTS, Janae | Wellington |
18.26 | Wellington HS Time Trials |
| 7 | WHITE, Ariana | Palm Beach Gardens |
18.70 | Dwyer Panther Open 2025 |
| 8 | CANELAS, Angelique | Atlantic Christian |
19.36 | Spanish River Invitational 2025 |