| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | West Boynton Middle School | A | Hinds | Darby | Bissoon | Brown | FS | |
| 2 | Don Estridge High Tech Middle Sc | A | 56.74 | |||||
| 3 | Osceola Creek Middle School | A | Williams | Jean | Hughes | Nosworthy | 56.12 | |
| 4 | Crestwood Community Middle Schoo | A | Lee | Legette | Moreland | Brown | 55.26 | |
| 5 | Omni Middle School | A | Jean-Baptiste | Cresspo | Ferrante | Guaglione | 55.61 | |
| 6 | Loggers' Run Middle School | A | 56.54 | |||||
| 7 | Wellington Landings Middle Schoo | A | Withrow | Cantero | Doris | Bianco | 56.97 | |
| 8 | Boca Raton Middle School | A | 1:00.04 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Christa McAuliffe Middle School | A | 54.27 | |||||
| 2 | John F. Kennedy Middle School | A | Lewis | Benton | Johnson | Wilkins | 54.19 | |
| 3 | Palm Springs Middle School | A | Taylor | Barrett | Telisca | Waters | 53.76 | |
| 4 | Roosevelt Middle School | A | Walker | Dykes | Pitts | Miller | 51.71 | |
| 5 | Howell L. Watkins Middle School | A | Johnson | Watkins | Hickson | Baptiste | 53.17 | |
| 6 | Bak Middle School of the Arts | A | Jefferson | Hopkins | Cambrone | Patillo | 54.11 | |
| 7 | Emerald Cove Middle School | A | DQ | |||||
| 8 | Woodlands Middle School | A | 54.30 |