| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Wellington |
A | Brooks | Lowe | Carbocci | Weir | 53.19 |
| 2 | Glades Central |
A | Dixon | Atkins | Gillyard | Stewart | 52.70 |
| 3 | King's Academy |
A | Foster | George | Crews | Hoxsie | 52.06 |
| 4 | Benjamin School |
A | Kujawa | Apple | Reddick | Dalton | 51.68 |
| 5 | Pahokee |
A | Jones | Pourch | Linder | Johnson | 51.72 |
| 6 | Spanish River |
A | Ulysse | Theissen | Durso | Okwor | 52.46 |
| 7 | Suncoast |
A | Bright | Hoim | Flore | Johnson | 53.09 |
| 8 | Lake Worth |
A | Henry | Vilmont | Hadley | Williams | DNS |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed |
|---|---|---|---|---|---|---|---|
| 1 | Palm Beach Gardens |
A | Andrews | Brown | Odita | Perry | 51.34 |
| 2 | Palm Beach Central |
A | Hendrix | Earle | Rejouis | Mindley | 50.23 |
| 3 | Seminole Ridge |
A | Gordon | James | Garced | Touma | 49.96 |
| 4 | American Heritage (DB) |
A | Milord | De Gruttola Aere | Johnson | Watts | 48.63 |
| 5 | Dwyer |
A | McClean | Crumbry | Bias | Mccalla | 49.79 |
| 6 | Atlantic (Delray) |
A | Austin | Murray | Davis | Waters | 50.03 |
| 7 | Park Vista |
A | Fleuricin | Bavaro | Brown | Surtain | 50.83 |
| 8 | Jupiter |
A | Monterroso | Granda | Norzea | Shaffer | 51.43 |