| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Glades Central |
A | Lafond | Kyles | Marshall | Meredith | 4:48.58 | FHSAA 2A District 8 |
| 4 | Pahokee |
A | Hamilton | White | Johnson | Johnson | 3:53.35 | FHSAA State Track & Field Championships (1A) |
| 5 | Royal Palm Beach |
A | Davis | Chapman | Neptune | Ennis | 4:16.09 | PBC Meet 2025 |
| 6 | Glades Day |
A | Lyles | Faragoi | Ferreira | Wilson | DNS | FHSAA 1A Region 4 |