| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Royal Palm Beach |
A | Desravins | Chapman | Neptune | St. Germain | 11:05.41 | FHSAA 4A Region 2 |
| 2 | John I. Leonard |
A | briceno | louis | fils-aime | toussaint | 11:54.84 | Pahokee Andre Powell Invitational |
| 3 | Glades Central |
A | Roa | Roa | Frazier | Patrick | 12:00.69 | Pahokee Anquan Boldin Invitational 2025 |
| 4 | Glades Day |
A | Lyles | Faragoi | Ferreira | Wilson | 12:59.21 | Glades Central Invite #1 2025 |