| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Royal Palm Beach |
A | Curry | Civil | Bowleg | Greenfield | 3:28.95 | FHSAA 4A District 8 |
| 4 | Glades Central |
A | Goring | Baptist | Paul | Georges | 3:22.26 | FHSAA State Track & Field Championships (2A/Para-Athlete) |
| 5 | Pahokee |
A | Logan | Jones | Stinfort | Morales | 3:22.80 | FHSAA State Track & Field Championships (1A) |