| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | ROA, Julianna | Glades Central |
6:35.30 | Royal Palm Beach Invitational 2026 |
| 2 | ROA, Julissa | Glades Central |
7:14.37 | John I Leonard Invitational 1 |
| 3 | MARTINEZ, Briana | Glades Day |
7:51.78 | John I Leonard Invitational 1 |
| 4 | HAYS, Elle | Glades Day |
9:29.77 | John I Leonard Invitational 1 |
| 5 | CRUZ, Leslie | Glades Central |
NT | |
| 6 | MCGLAMORY, Jaylen | Glades Day |
NT | |
| 7 | POOLE, Paylyn | Glades Day |
NT | |
| 8 | ROMAN, Carolina | Glades Central |
NT |