| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 3 | MEREDITH, D'Yami | Glades Central |
NT | |
| 4 | FORTESTA, Suze | Glades Central |
31.60 | Royal Palm Beach Invitational 2026 |
| 5 | LOUIS, jenny | John I. Leonard |
NT | 20.09 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | DEMPSEY, Cailey | Pahokee |
20.91 | John I Leonard Invitational 1 |
| 3 | LAFOND, Raymeshia | Glades Central |
20.57 | Royal Palm Beach Invitational 2026 |
| 4 | BYTHWOOD, De'Asia | Pahokee |
20.01 | Royal Palm Beach Invitational 2026 |
| 5 | GORDON, Yoriah | Glades Central |
20.39 | Royal Palm Beach Invitational 2026 |
| 6 | POOLE, Paylyn | Glades Day |
20.69 | Kings Academy Varsity 4 Sanctioned |
| 7 | SONSON, Kelis | Pahokee |
22.89 | Royal Palm Beach Invitational 2026 |