| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Village School |
A | 10:53.52 | FHSAA 1A District 12 |
| 2 | Donahue Academy |
A | 12:09.42 | CSN Invitational Powered by UnderArmour (Qualifier) |
| 3 | Mason Classical Academy |
A | 13:00.55 | Guy Thomas Memorial Classic (Approved Qualifier) |