| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | DAVID, Namiah | Naples Classical | NT | |
| 3 | TANELI, Tiana | Naples Classical | NT | |
| 4 | MOLLER, Luciana | Donahue Academy |
1:14.74 | 2026 South Fort Myers Wolfpack Invitational |
| 5 | FAJARDO, Salome | Donahue Academy |
1:09.44 | CSN Invitational Powered by UnderArmour (Qualifier) |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | REILY, Leah | Naples Classical | NT | |
| 2 | GUIDRY, Aubrey | Marco Island Academy |
1:25.51 | CSN Invitational Powered by UnderArmour (Qualifier) |
| 3 | SZCZEPKOWSKI, Charlotte | Naples Classical | NT | |
| 4 | FELDMAN, Gia | Village School |
1:04.11 | 2026 South Fort Myers Wolfpack Invitational |
| 5 | RAMIREZ, Sophia | Naples Classical | NT | |
| 6 | ROSARIO, D'yana | Mason Classical Academy |
NT | |
| 7 | GUALARIO, Alli | Village School |
1:15.88 | 2025 PCAC Championship |
| 8 | PIERRE LOUIS, Allichard | Mason Classical Academy |
NT |