| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Mason Classical Academy |
A | Demezier | Finn | Koscica | Patel | 4:51.46 | Palmetto Ridge Bear Classic |
| 4 | Village School |
A | Garner | Gualario | Dooney | Feldman | 4:24.48 | FHSAA 1A Region 3 |
| 5 | Donahue Academy |
A | Moller | Shaw | Green | Fajardo | 4:40.18 | 62nd Eagle Invitational |