| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Village School |
A | Garner | Sullivan | Gualario | Lappin | 10:53.52 | FHSAA 1A District 12 |
| 2 | Donahue Academy |
A | Graham | Fulmer | Fulmer | Graham | 12:09.42 | CSN Invitational Powered by UnderArmour (Qualifier) |
| 3 | Mason Classical Academy |
A | Finn | Koscica | Patel | Petit Frere | 13:00.55 | Guy Thomas Memorial Classic (Approved Qualifier) |