| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | BIDWELL, Eliza | Village School |
1:30.14 | 2025 South Fort Myers Wolfpack Invitational |
| 3 | VLAHAKIS, Mia | Mason Classical Academy |
1:24.08 | Patriots Friday Night Lights |
| 4 | ROOK, Isabella | Donahue Academy |
1:12.63 | Cypress Panther Invitational |
| 5 | OCTELUS, Alyiaa | Mason Classical Academy |
1:23.36 | Mark McGarity Freshman/Sophomore meet |
| 6 | RITTER, Francesca | Donahue Academy |
1:25.74 | Cypress Panther Invitational |
| 7 | GREEN, Nina | Donahue Academy |
1:32.02 | Village School Home Meet |