| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | RITTER, Francesca | Donahue Academy |
21.65 | Cypress Panther Invitational |
| 3 | VLAHAKIS, Mia | Mason Classical Academy |
21.25 | Palmetto Ridge Bear Classic |
| 4 | ROOK, Isabella | Donahue Academy |
17.39 | April Fools Day Golden Gate Mini Meet #4, |
| 5 | BIDWELL, Eliza | Village School |
19.98 | Patriots Friday Night Lights |
| 6 | GREEN, Nina | Donahue Academy |
21.52 | Cypress Panther Invitational |