| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 3 | MONDESIR, Micheguestey | Palm Beach Lakes | NT | 23.58 |
| 4 | ST. JULIEN, Alex | Dwyer | NT | NT |
| 5 | MADDEN, Reece | Jupiter | NT | NT |
| 6 | JOHNS, Giovanni | Palm Beach Lakes | NT | 23.56 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | HOWARD, Teyshaun | Palm Beach Lakes | NT | 24.5 |
| 2 | THOMAS, Malachi | Jupiter | NT | NT |
| 3 | BALFOUR, Kaden | Suncoast | 26.23 | Jupiter Preseason 2023 |
| 4 | FLANDERS, Derrick | Dwyer | 24.96 | PBGHS Gator Invitational 2023 |
| 5 | PLUMMER, Jamaul | Palm Beach Lakes | 25.90 | PBGHS Gator Invitational 2023 |
| 6 | RICHARDSON, Tony | Suncoast | NT | NT |
| 7 | VILLANUEVA, Christian | Dwyer | NT | NT |
| 8 | GONSALVES, Carlos | Suncoast | NT | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | LUBIN, David | Palm Beach Gardens | 24.22 | PBGHS Gator Invitational 2023 |
| 2 | DALEY, Dean | Palm Beach Gardens | 23.64 | The Ellis Elite 16 Track & Field Invitational |
| 3 | LETTINGHAM, Jajuan | Dwyer | 23.12 | Calvary Christian FtL Annual Invitational |
| 4 | OWENS, William | Jupiter | 22.39 | Panther Relays 2023 |
| 5 | BAKER, Jayden | Jupiter | 22.94 | Panther Relays 2023 |
| 6 | SCOTT, Kameron | Suncoast | 23.61 | Warrior Invitational 2023 |
| 7 | METAYER, Isaiah | Palm Beach Gardens | 23.98 | PBGHS Gator Invitational 2023 |
| 8 | MALCOLM, Aiden | Palm Beach Gardens | 24.74 | Panther Open |