| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Loggers' Run Middle School | A | 51.04 | |||||
| 2 | Christa McAuliffe Middle School | A | 50.26 | |||||
| 3 | Okeeheelee Middle School | A | Martinez | Parra Leblanc | Perez Martinez | Clairvoyant | 49.99 | |
| 4 | Bak Middle School of the Arts | A | Baker | Thompson | Lindor | Bodden | 49.63 | |
| 5 | West Boynton Middle School | A | Shalom | Nembhard | Thomas | Pierre | 49.73 | |
| 6 | Delray Beach Carver Middle Schoo | A | Dieujuste | Charleston | Alexander | Janvier | 50.12 | |
| 7 | Palm Springs Middle School | A | Joaseus | Simmons | Ruiz | Pugh | DQ | |
| 8 | Eagles Landing Middle School | A | 51.57 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Roosevelt Middle School | A | Johnson | Christie | Dent | Foster | 48.64 | |
| 2 | Howell L. Watkins Middle School | A | Creary | Simpson | Jerome | Campbell | 48.13 | |
| 3 | John F. Kennedy Middle School | A | Reed | Powell | Moreau | Bell | 47.77 | |
| 4 | Emerald Cove Middle School | A | 46.42 | |||||
| 5 | Osceola Creek Middle School | A | Archambault | Jimenez | Machin | Prince | 47.17 | |
| 6 | Crestwood Community Middle Schoo | A | Colins | Perissien | Lewis | Altidor | 48.12 | |
| 7 | Woodlands Middle School | A | Burrows | Johns | Pratt | Troupe | 48.56 | |
| 8 | Don Estridge High Tech Middle Sc | A | 49.36 |