| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Palm Beach Gardens |
A | Voltaire | Newson | Alexander | Coicou | DNS | PBGHS Invitational 2025 |
| 2 | Jupiter |
A | Ward | Grashof | Jackson | Morgan | 3:35.10 | Bulldog Invitational 2025 |
| 3 | Atlantic (Delray) |
A | Scruggs | Dean | Davis | Horne | 3:30.67 | Cardinal Newman Invitational 2025 |
| 4 | Park Vista |
A | Almonor | Luong | Pinal | Torrijos | 3:29.92 | Panther Relays 2025 |
| 5 | Royal Palm Beach |
A | Murvin | murvin | Franceour | Thomas | 3:30.04 | PBGHS Invitational 2025 |
| 6 | Palm Beach Central |
A | Atkins | Ledford | Feaman | Schry | 3:32.70 | Panther Relays 2025 |
| 7 | Dwyer |
A | Quince | Price | Augustin | Williams | 3:35.55 | Bob Hayes High School Invitational |
| 8 | American Heritage (DB) |
A | St Julien | Gibbons | Liberzon | Steele | DNS | Panther Relays 2025 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Santaluces |
A | Sanon | Zennot | Gerald | Michel | 3:27.11 | Panther Relays 2025 |
| 2 | Spanish River |
A | Terentis | Toscano | Cescato | Reyes | 3:26.29 | 2025 Coral Springs Invitational |
| 3 | Pahokee |
A | Crawford | Bryant | Edward | Jones | 3:25.14 | Glades Central Invite #1 2025 |
| 4 | Cardinal Newman |
A | Hughley | Licata | Kissel | Tuma | DNS | Southridge Spartan Invitational |
| 5 | Benjamin School |
A | Sukhu | Ryba | Faniel | Winfield | 3:23.56 | Benjamin Invite #1 2025 (Sanctioned) |
| 6 | King's Academy |
A | Stewart | Kakuk | Schmidtke | Hunter | 3:25.45 | Calvary Christian FTL Varsity Invitational |
| 7 | Glades Central |
A | Goring | Stinfort | Ray | Barnes | 3:27.04 | Glades Central Invite #1 2025 |
| 8 | Wellington |
A | Spence | Paul | Smith | Borgella | 3:27.43 | Cardinal Newman Invitational 2025 |