| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | TERENTIS, Ryan | Spanish River |
50.90 | 2025 Coral Springs Invitational |
| 2 | DAMASCENO, Fernando | Boca Raton |
50.87 | Cardinal Newman Invitational 2025 |
| 3 | GRANTLIN, Yohann | Inlet Grove |
50.25 | Atlantic HS Invitational 2 |
| 4 | ANTONUCCI, Peter | FAU High |
50.02 | Pine Crest Varsity Open |
| 5 | MONTELUS, karlvin | John I. Leonard |
50.24 | Atlantic HS Invitational 2 |
| 6 | BELL, Xzavier | Lake Worth |
50.30 | Cardinal Newman Invitational 2025 |
| 7 | VOLTAIRE, Josiah | Palm Beach Gardens |
50.89 | Cardinal Newman Invitational 2025 |
| 8 | WARD, Simon | Dr. Joaquin Garcia | 51.06 | Spanish River Invitational 2025 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | MICHEL, Dylan | Santaluces |
49.73 | Glades Central Invite #1 2025 |
| 2 | WINFIELD, Michael | Benjamin School |
49.45 | Kings Academy Varsity 2 |
| 3 | STEWART, Tristan | King's Academy |
48.64 | Calvary Christian FTL Varsity Invitational |
| 4 | MILLER, Jackson | Cardinal Newman |
47.91 | 2025 The Ellis Elite 16 Track and Field Invitational |
| 5 | HORNE, Tavius | Atlantic (Delray) |
48.58 | Spanish River Invitational 2025 |
| 6 | PAUL, Prince | Wellington |
49.30 | Jupiter Warrior Invitational 2025 |
| 7 | JONES, Da'Corrian | Pahokee |
49.60 | Glades Central Invite #1 2025 |
| 8 | LEDFORD, Pierson | Palm Beach Central |
49.98 | Wellington HS Invitational |