| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | JONES, Jade | King's Academy |
26.83 | Calvary Christian FTL Varsity Invitational |
| 2 | WATTS, Kamaya | American Heritage (DB) |
26.65 | Dr Garcia Invite #1 2025 |
| 3 | ARP, Mary | Suncoast |
26.25 | Clyde Harris Invitational at |
| 4 | MURRAY, Jamiyah | Atlantic (Delray) |
25.94 | John I Leonard Invitational |
| 5 | GIBSON, Arielle | Inlet Grove |
26.07 | Cardinal Newman Invitational 2025 |
| 6 | NOEL, Arya | FAU High |
26.61 | Calvary Christian FTL Varsity Invitational |
| 7 | JULIEN-JEUNE, Eranaelle | West Boca Raton |
26.73 | Park Vista #1 2025 |
| 8 | RAZZ, Rahniya | Lake Worth |
26.90 | Cardinal Newman Invitational 2025 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | PERRY, A'cian | Palm Beach Gardens |
25.55 | PBGHS Invitational 2025 |
| 2 | AUGUSTIN, Ketteline | John I. Leonard |
25.10 | Glades Central Invite #1 2025 |
| 3 | RICHARDSON, Jamaria | Pahokee |
24.35 | Glades Central Invite #1 2025 |
| 4 | MILORD, Jailyn | FAU High |
23.71 | Pine Crest Varsity Open |
| 5 | FORBES, E'tesia | Glades Central |
24.16 | Glades Central Invite #1 2025 |
| 6 | MARSHALL, Akari | Benjamin School |
24.95 | Kings Academy Varsity 2 |
| 7 | JOHNSON, Jordan | Pahokee |
25.28 | Glades Central Invite #1 2025 |
| 8 | JEAN, Giselene | Boynton Beach |
25.84 | Clyde Harris Invitational at |