| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | BROOKS, Cailyx | Wellington |
12.92 | Cardinal Newman Invitational 2025 |
| 2 | GRANDA, Gianna | Jupiter |
12.88 | Benjamin Invite #2 2025 (Sanctioned) |
| 3 | WATTS, Kamaya | American Heritage (DB) |
12.71 | Dr Garcia Invite #1 2025 |
| 4 | AUGUSTIN, Ketteline | John I. Leonard |
12.57 | Pahokee Andre Powell Invitational |
| 5 | JEAN, Jeanissa | Dwyer |
12.70 | Cardinal Newman Invitational 2025 |
| 6 | MILORD, Julian | American Heritage (DB) |
12.77 | Wellington HS Invitational |
| 7 | JONES, Bryanna | Lake Worth |
12.90 | Cardinal Newman Invitational 2025 |
| 8 | JOHNSON, Ameenia | Suncoast |
12.94 | PBGHS Invitational 2025 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | ARP, Mary | Suncoast |
12.56 | PBGHS Invitational 2025 |
| 2 | PERRY, A'cian | Palm Beach Gardens |
12.47 | PBGHS Invitational 2025 |
| 3 | MARSHALL, Akari | Benjamin School |
11.95 | Benjamin Invite #2 2025 (Sanctioned) |
| 4 | FORBES, E'tesia | Glades Central |
11.83 | Pahokee Andre Powell Invitational |
| 5 | MURRAY, Jamiyah | Atlantic (Delray) |
11.90 | 2025 Coral Springs Invitational |
| 6 | MILORD, Jailyn | FAU High |
12.14 | St Andrews Varsity |
| 7 | GIBSON, Arielle | Inlet Grove |
12.49 | Pahokee Andre Powell Invitational |
| 8 | RAZZ, Rahniya | Lake Worth |
12.57 | Cardinal Newman Invitational 2025 |