| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Clewiston |
B | McKire | Terry | Bryant | Jeudy | 43.54 | Sebring Meet #2 2025 |
| 2 | Okeechobee |
B | Pineda | Lopez-Trimino | Crum | Rogers | 45.29 | Sebring Relay Meet 2025 |
| 3 | LaBelle |
A | Rivas | Hernandez | Davis | Bradshaw | 44.95 | Sebring Meet #2 2025 |
| 4 | Okeechobee |
A | Campbell | Hamilton | Evans | Brifil | 45.29 | Sebring Relay Meet 2025 |
| 5 | Moore Haven |
A | Gunsby | Ammons | Askew | Adderley | 44.24 | Sebring Meet #2 2025 |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | Clewiston |
A | Mason | Moody | Harshaw | Pope | DNS | Sebring Meet #2 2025 |