| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | PEREZ, Aliyah | Moore Haven |
32.93 | 2025 South Fort Myers Wolfpack Invitational |
| 3 | HEBEL-HOOVER, Island | Okeechobee |
29.98 | Satellite High Reggie Johnson Invite |
| 4 | BARRETT, Amiya | Okeechobee |
31.23 | Satellite High Reggie Johnson Invite |
| 5 | BREDWOOD, Cambria | Clewiston |
NT | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | WILLIAMS, Jayla | LaBelle |
29.19 | Immokalee Last Chance |
| 2 | STEPHENS, Jahpheria | Clewiston |
29.06 | Lake Placid Invite |
| 3 | MCCAIN, Shania | Clewiston |
28.74 | 65th Annual Edison Relays |
| 4 | SANDOVAL, Valerie | LaBelle |
28.77 | 65th Annual Edison Relays |
| 5 | HERRERA, Eliana | Moore Haven |
29.15 | Immokalee Last Chance |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | NESBY, Ciarah | Okeechobee |
28.64 | Royal Palm Beach Invitational 2025 |
| 2 | SANDOVAL, Anakaren | LaBelle |
28.25 | Sebring Meet #2 2025 |
| 3 | LING, Akeelah | Moore Haven |
25.24 | Sebring Meet #2 2025 |
| 4 | ROCHELLE, Jayda | Okeechobee |
27.95 | Jensen Beach Invitational 2025 |
| 5 | WHEATLE, Asania | LaBelle |
28.35 | Lake Placid Invite |