| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Lake Placid |
A | Dillon | Carlini-Smith | Aboytes | Henry | DNS | Avon Park Red Devil Invitational |
| 2 | Moore Haven |
A | Myers | Faison | Herrera | Myers | 52.08 | Avon Park Chet Brojek Track Invitational |
| 3 | Glades Central |
A | Josinvil | Forbes | Slaughter | Marshall | 48.57 | 2026 PBC County Championships |
| 4 | LaBelle |
A | Williams | Wheatle | Sandoval | Jackson | DNF | Lake Placid Invitational 2026 |
| 5 | Moore Haven |
B | Bowers | Santana | Green | Ash | DNF | TFRRS 03/31/26 - Throwers |