| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | LaBelle |
A | Chairez | Johnson | Walker | Lewis | 11:21.06 | Charlotte Invitational |
| 2 | Glades Central |
A | Patrick | Roa | Roman | Roa | 11:56.39 | Dr. Garcia Meet #3 2026 |
| 3 | Clewiston |
A | Emran | Lopez | Wright | Rivers | 15:29.32 | Lake Placid Invitational 2026 |
| 4 | DeSoto County |
A | Martinez | Rios | Trejo-Balderas | Naro | DNS |